METHODS IN ENZYMOLOGY VOL.522. MODELING, ACTIVATION, INTERACTIONS AND VIRTUAL SCREENING

The Protein Local Optimization Program and G-Protein Coupled Receptors: Loop Restoration and Applications to Homology Modeling

Dahlia A. Goldfeld and Richard A. Friesner


Modeling Active GPCR Conformations

Bruck Taddese, Lisa M Simpson, Ian D Wall, Frank E Blaney and Christopher A Reynolds


Molecular Modeling of Adenosine Receptors

Adriano Martinelli and Gabriella Ortore


Predicting G Protein-Coupled Receptors Families using Different Physiochemical Properties and Pseudo Amino Acid Composition

Zia-ur-Rehman, Muhammad Tayyeb Mirza, Asifullah Khan, Henri Xhaard


Computational Design and Experimental Characterization of GPCR Segment Models

Abby L. Parrill

GPCR Interactions with Other Molecules and Cross-Talk


G Protein-Coupled Receptor Interaction with Small GTPases

Chunmin Dong and Guangyu Wu


Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G Protein Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA)

Annie Barbeau, Antoine G. Godin, Jody L. Swift, Yves De Koninck, Paul W. Wiseman and Jean-Martin Beaulieu


NMR Methods for Detection of Small Molecule Binding to RGS4

Andrew J. Storaska and Richard R. Neubig


Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-Protein Regulatory Motif in the Intact Cell

Sukru Sadik Oner, Joe B. Blumer and Stephen M. Lanier


Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors

Adam M. Knepp, Thomas P. Sakmar, and Thomas Huber


cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors

Jesper Mosolff Mathiesen, Line Vedel and Hans Bräuner-Osborne


Rhodopsin-Lipid Interactions Studied by NMR

Olivier Soubias and Klaus Gawrisch

GPCR Bias


Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity

Kathryn M. Appleton, Mi-Hye Lee, Christian Alele, Christine Alele, Deirdre K. Luttrell, Yuri K. Peterson, Thomas A . Morinelli, and Louis M. Luttrell

Virtual Screening


Application of Monte Carlo-based Receptor-Ensemble Docking to Virtual Screening for GPCR Ligands

Santiago Vilar and Stefano Costanzi


From Heptahelical Bundle to Hits from the Haystack: Structure-Based Virtual Screening for GPCR Ligands

A.J. Kooistra, L. Roumen, R. Leurs, I.J.P. de Esch, C. de Graaf

GPCR Activation


Kinetics and Dynamics in the G-Protein Coupled Receptor Signaling Cascade

Jean-Pierre Vilardaga, Timothy N. Feinstein, Vanessa L. Wehbi, and Guillermo Romero


Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors

Joseph Goncalves, Markus Eilers, Kieron South, Chikwado A. Opefi, Philippe Laissue, Philip J. Reeves and Steven O. Smith


Post-Translation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism

Hui Zheng, Horace H. Loh and Ping-Yee Law


Protein S-Nitrosylation Measurement

Yu Qin, Anindya Dey and Yehia Daaka

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors.

Features:
Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers G protein coupled receptors
Contains chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.