METHODS IN ENZYMOLOGY VOL.521. TRAFFICKING AND OLIGOMERIZATION

Therapeutic Rescue of Misfolded/Mistrafficked Mutants: Automation-Friendly High Throughput Assays for Identification of Pharmacoperone Drugs of GPCRs

David C. Smithson, Jo Ann Janovick, P. Michael Conn


Trafficking of the Follitropin Receptor

Alfredo Ulloa-Aguirre, James A. Dias, George Bousfield, Ilpo Huhtaniemi, and Eric Reiter


Single Molecule Imaging Technique to Study the Dynamic Regulation of GPCR Function at the Plasma Membrane

B.E. Snaar-Jagalska, A. Cambi, T. Schmidt and S. de Keijzer


GPCR Oligomerization and Receptor Trafficking

Richard J. Ward, Tian-Rui Xu and Graeme Milligan


b-Arrestins and G Protein-Coupled Receptor Trafficking

Dong Soo Kang, Xufan Tian, and Jeffrey L. Benovic


Tracking Cell Surface Mobility of GPCRs Using a-Bungarotoxin Linked Fluorophores

Saad Hannan, Megan E Wilkins, P Thomas and Trevor G Smart


Regulatory Mechanism of G Protein-Coupled Receptor Trafficking to the Plasma Membrane: A Role for mRNA Localization

Kusumam Joseph, Eleanor K. Spicer and Baby G. Tholanikunnel


Dissecting Trafficking and Signaling of Atypical Chemokine Receptors

Elena Borroni, Cinzia Cancellieri, Massimo Locati, Raffaella Bonecchi


Trafficking Motifs


Systematic and Quantitative Analysis of G Protein-Coupled Receptor Trafficking Motifs

Carl M. Hurt, Vincent K. Ho, and Timothy Angelotti


Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors

Guangyu Wu


Amino Acid Residues of G-Protein Coupled Receptors Critical for Endoplasmic Reticulum Export and Trafficking

Motonao Nakamura, Daisuke Yasuda, Nobuaki Hirota, Teruyasu Yamamoto, Satoshi Yamaguchi, Takao Shimizu and Teruyuki Nagamune


GPCR Oligomerization


G Protein-Coupled Heteromers Regulation in Disease

Ivone Gomes, Achla Gupta and Lakshmi A. Devi


Hetero-Oligomerization and Specificity Changes of G Protein-Coupled Purinergic Receptors: Novel Insight into Diversification of Signal Transduction

Tokiko Suzuki, Kazunori Namba, Natsumi Mizuno and Hiroyasu Nakata


Bimolecular Fluorescence Complementation Analysis of G Protein-Coupled Receptor Dimerization in Living Cells

Karin F.K. Ejendal, Jason M. Conley, Chang-Deng Hu, and Val J. Watts


G-Protein Coupled Receptor Heterodimerization in the Brain

Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Pere Garriga, Francisco Ciruela, Manuel Narvaez, Alexander O. Tarakanov, Miklós Palkovits, Luigi F. Agnati and Kjell Fuxe


Experimental Strategies for Studying G Protein-Coupled Receptor Homo- and Heteromerization with Radioligand Binding and Signal Transduction Methods

Roberto Maggio, Cristina Rocchi, and Marco Scarselli


Analysis of GPCRs Dimerization Using Acceptor Photobleaching Resonance Energy Transfer Techniques

Marta Busnelli, Mario Mauri, Marco Parenti and Bice Chini


Techniques for the Discovery of GPCR-Associated Protein Complexes

Avais Daulat , Pascal Maurice and Ralf Jockers


Expression, Purification and Analysis of GProtein-Coupled Receptor Kinases

Rachel Sterne-Marr, Alison I. Baillargeon, Kevin R. Michalski, and John J. G. Tesmer


Modern Methods to Investigate the Oligomerization of Glycoprotein Hormone Receptors (TSHR,LHR, FSHR)

Marco Bonomi and Luca Persani

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors.

Features:
Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers G protein coupled receptors
Contains chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high-throughput assays for identifying pharmacoperone drugs