TECHNOLOGY PLATFORMS FOR 3D CELL CULTURE: A USER´S GUIDE

1 An introduction to the third dimension for routine cell culture 1

• Part I: Aggregate ]based technologies
2 Gravity ]enforced microtissue engineering 23
3 Physiologically relevant spheroid models for three ]dimensional cell culture 50
4 NanoCulture Plate: A scaffold ]based high ]throughput three ]dimensional cell culture system suitable for live imaging and co ]culture 74
5 Micro ]moulded non ]adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish® 97
6 Organotypic microtissues on an air ]liquid interface 123

• Part II: Hydrogels
7 Materials and assay systems used for three ]dimensional cell culture 145
8 HyStem® a customisable hyaluronan ]based hydrogel matrix for 3D cell culture 173
9 3 ]D Life biomimetic hydrogels: A modular system for cell environment design 197

• Part III: Scaffolds
10 Alvetex® a highly porous polystyrene scaffold for routine three ]dimensional cell culture 225
11 CelluSponge™ and Go Matrix as innovative three ]dimensional cell culture platforms 250
12 Mimetix® electrospun scaffold: An easy ]to ]use tool for 3D cell culture in drug discovery and regenerative medicine 284

• Part IV: 3D bioreactor technologies
13 Quasi Vivo® bioreactor technology 305
14 Three ]dimensional cell ]based assays in hollow fibre bioreactors 327
15 Three ]dimensional engineered tissues for high ]throughput compound screening: Mechanical properties of skin and ageing 351
16 Three ]dimensional cell culture in the Rotary Cell Culture System™ 370

Glossary 386
Index 393

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.

• Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture
• Features contributions from leading developers and researchers active in 3D cell technology
• Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied
• Includes information on resources and technical support to help initiate the use of 3D culture methods

Author Information
Stefan Przyborski is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.