STANDARD OPERATIONAL PROCEDURES IN REPRODUCTIVE MEDICINE: LABORATORY AND CLINICAL PRACTICE

• LABORATORY PROCEDURES
1 The documentation system
2 Culture Media Techniques
3 IVF incubator handling
4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC)
5 Semen collection and sample reception
6 Spermiogramm, Makler chamber, Neubauer chamber, CASA
7 Sperm viability testing
8 Sperm preparation – Swim up, density gradient, migration chamber, zeta method
9 Sperm selection using hyaluronan binding
10 IVF - Insemination
11 ICSI
12 Oocyte in vitro maturation – Isolation of COCs and culture for maturation
13 Artificial oocyte activation
14 Cryopreservation - Sperm TESE and MESA
15 Vitrification – Oocytes, 2PN, Embryos, Blastocysts
16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts
17 Oocyte selection
18 Zygote selection
19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6
20 Time-lapse imaging
21 Assisted hatching
22 Zona manipulation at various embryo stages: Mechanical / acidic medium
23 Biopsy: polar body, blastomere, trophectoderm
24 Sampling of cells after biopsy for aCGH
25 Embryo transfer – standard and ultrasound guided
26 Hygenic standards in the IVF laboratory
27 Quality control of IVF laboratory supplies – Mouse Embryo Assay (MEA)
28 Troubleshooting in the IVF laboratory
29 Microscopy
30 Fertility preservation
• CLINICAL PROCEDURES
31 Ultrasound evaluation in treatment management
32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies
33 Infertility ultrasound evaluation: One Stop Procedure
34 Office Hysteroscopy
35 Hysteroscopic management of uterine septum
36 Hysteroscopic Lysis of Intrauterine Adhesions
37 Hysteroscopic Management of Submucosal Fibroids
38 Saline infusion sonohysterography
39 General Principles of Laparoscopy
40 Microlaparoscopy
41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy
42 Laparoscopic Excision of a Large Endometrioma
43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosis
44 Laparoscopic management of mature cystic teratoma
45 Tubal Cannulation for Proximal Tubal Disease
46 Robotic assisted laparoscopic myomectomy
47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization
48 Unexplained infertility
49 Male Evaluation for Infertility
50 Hysterosalpingography
51 Intrauterine insemination: clinical perspectives
52 GnRH agonist to trigger final follicular maturation in IVF
53 GnRH agonist Protocols
54 GnRH agonists before IVF for advanced endometriosis
55 Microdose GnRH Agonist Flare Protocols
56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization
57 Minimal stimulation for IVF
58 Ovarian Hyperstimulation Syndrome
59 The Prediction of OHSS
60 Tips on technique of embryo transfer
61 Difficult Embryo Transfers
62 In vitro maturation of oocytes for fertility treatment and fertility preservation
63 Complications and outcomes of IVF
64 Obstetric complications after in-vitro fertilization
65 Twins pregnancy after ART
66 IVF and endometriosis
Graciela Kohls Ilgner and Juan Antonio Garcia Velasco
67 Leiomyoma of the Uterus
68 Office Management of Polycystic Ovarian Syndrome
69 Adenomyosis: Imaging and Treatment
70 Ectopic Pregnancy: Evaluation and Management

Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues.

Features
• Offers a cookbook approach to operational procedures
• Covers both laboratory and clinical procedures
• Benefits both starters and experienced staff
• Provides an easy layout to take in the whole procedure at a glance
• Contains the contributions of experts in the area

Authors
• Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA
• Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany