PATHOGENIC YERSINIA. METHODS AND PROTOCOLS

An intradermal model for Yersinia pestis inoculation
Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis
Standardized method for aerosol challenge of rodents with Yersinia pestis for modeling primary pneumonic plague
Mouse Models of Yersiniosis
Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-transmitted Yersinia pestis
Laser scanning microscopy of Yersinia pestis infected tissues
Real-time monitoring of Yersinia pestis promoter activity by bioluminescence imaging
Discovering Yersinia-Host Interactions by Tissue Dual RNA-seq
Detection of cells translocated with Yersinia Yops in infected tissues using ß-lactamase fusions
Monitoring of neutrophil recruitment to mice lungs during pneumonic plague
Co-infection assay to determine Yersinia pestis competitive fitness in fleas
Drosophila as a model for understanding the insect host of Yersinia pestis
Intracellular assays to monitor survival and growth of Yersinia pestis within macrophages
Human neutrophil isolation and degranulation responses to Yersinia pestis infection
Measurement of Yersinia translocon pore formation in erythrocytes
Analysis of inflammasome activation in response to Yersinia infection by fluorescence microscopy detection of active caspase-1 puncta
Methods for detection of pyrin inflammasome assembly in macrophages infected with Yersinia spp.

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Features
• Includes cutting-edge techniques
• Provides step-by-step detail essential for reproducible results
• Contains key implementation advice from the experts