ISOTOPE LABELING OF BIOMOLECULES – LABELING METHODS. METHODS IN ENZYMOLOGY, VOL. 565

Isotope Labeling of Biomolecules - Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules.

Key Features
- Contains contributions from leading authorities in the field of isotope labeling of biomolecules
- Informs and updates on the latest developments in the field
- Provides comprehensive information on stable isotope labeling methods and applications for biomolecules

Readership
Scientists interested in using stable isotope labeling for structural determination (i.e., SANS and NMR) and other applications (i.e., MS and HDX).

Author
Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.

Contents
1. Robust High Yield Methodologies for 2H and 2H/15N/13C Labelling of Proteins for Structural Investigations using Neutron Scattering and NMR
2. Protein labeling in Escherichia coli with 2H, 13C and 15N
3. Escherichia Coli Auxotroph Host Strains for Amino Acid-Selective Isotope Labeling of Recombinant Proteins
4. 19F-Modified Proteins and 19F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions
5. Biopolymer Deuteration For Neutron Scattering and Other Isotope-Sensitive Techniques
6. Production of Bacterial Cellulose with Controlled Deuterium-Hydrogen Substitution for Neutron Scattering Studies
7. Isotopic Labeling of Proteins in Halobacterium Salinarum
8. Amino Acid Selective Unlabeling in Protein NMR Spectroscopy
9. Isotope Labeling of Eukaryotic Membrane Proteins in Yeast for Solid-State NMR
10. Development of Approaches for Deuterium Incorporation in Plants
11. Isotope labeling of proteins in insect cells
12. Effective Isotope Labeling of Proteins in a Mammalian Expression System
13. Escherichia Coli Cell-Free Protein Synthesis and Isotope Labeling of Mammalian Proteins
14. Rapid Biosynthesis of Stable Isotope Labeled Peptides from a Reconstituted in Vitro Translation System for Targeted Proteomics
15. Labeling of Membrane Proteins by Cell-Free Expression
16. Selective Amino Acid Segmental Labeling of Multi-Domain Proteins
17. Labeling Monosaccharides with Stable Isotopes