INDUCTION OF BONE FORMATION IN PRIMATES: THE TRANSFORMING GROWTH FACTOR-BETA 3

Introduction: The Discovery of Redundancy and the Bone Induction Prerogative of the Three Mammalian Transforming Growth Factor-ß Isoforms
Ugo Ripamonti
Induction of Bone Formation before Redundancy and the Discovery of the Induction of Bone Formation by the Mammalian Transforming Growth Factor-ß Isoforms

Regenerative Medicine, the Induction of Bone Formation, Bone Tissue Engineering, and the Osteogenic Proteins of the Transforming Growth Factor-ß Supergene Family
Ugo Ripamonti
Regenerative Medicine and Bone: Formation by Autoinduction
Induction of Bone Formation: Osteogenesis in Angiogenesis
Homologous but Molecularly Different Pleiotropic Proteins of the TGF-ß Supergene Family Initiate Endochondral Bone Formation

Rapid Induction of Bone Formation by the Transforming Growth Factor-ß3Isoform
Ugo Ripamonti
Transforming Growth Factor-ß3 Isoform
TGF-ß3 Isoform and the Induction of Bone Formation in Primates
Coral-Derived Mascroporous Bioreactor as Delivery System for the Biological Activity of the Recombinant hTGF-ß3 Isoform
hTGF-ß3 Master Gene and Gene Product: Transfiguration of Neoplastic Masses into Bone

Induction of Bone Formation by the Mammalian Transforming Growth Factor-ßs: Molecular and Morphological Insights
Raquel Duarte, Kurt Lightfoot, and Ugo Ripamonti
Introduction
Role of TGF-ß in Differentiation of Osteoblasts
Role of TGF-ß3 in Bone Formation: Lessons from the Nonhuman Primate Papio ursinus
Perspectives

Regeneration of Mandibular Defects in Nonhuman Primates by the Transforming Growth Factor-ß3 and Translational Research in Clinical Contexts
Ugo Ripamonti and Carlo Ferretti
Tissue Induction and Regeneration of Craniomandibulofacial Defects
Rapid Induction of Bone Formation by the hTGF-ß3 Osteogenic Device in Mandibular Defects of the Chacma Baboon (P. ursinus)

Synergistic Induction of Bone Formation by Relatively Low Doses of Transforming Growth Factor-ß1 and -ß3 in Binary Application with Recombinant Human Osteogenic Protein-1
Ugo Ripamonti
Homologous but Molecularly Different Multiple Isoforms of the TGF-ß Supergene Family initiate the Induction of Bone Formation in Primate Species
Tissue Morphogenesis and Synergistic Interaction by Interposed Homologous Recombinant Morphogens
Synergistic Induction of Bone Formation
Temporal Expression of Markers of Bone Differentiation by Induction: Mechanistic Insights into the Synergistic Induction of Bone Formation
Synergistic Induction of Bone Formation in Full-Thickness Mandibular Defects of P. ursinus

Induction of Periodontal Tissue Regeneration by Recombinant Human Transforming Growth Factor-ß3 with and without Myoblastic/Pericytic Stem Cells in Papio ursinus
Jean-Claude Petit and Ugo Ripamonti
Introduction
Origins
Periodontal Tissue Regeneration by the Osteogenic Proteins of the TGF-ß Supergene Family
Periodontal Tissue Regeneration by Naturally Derived, Highly Purified and Recombinant Human Bone Morphogenetic Proteins
Induction of Periodontal Tissue Regeneration by Recombinant Human TGF-ß3 in P. ursinus
Challenges and Perspectives in Regenerative Medicine of the Periodontal Tissues by the hTGF-ß3 Isoform

• Covers the isolation and cellular biology of the TGF-ß3 isoform
• Looks at the redundancy of bone induction signaling and the synergies between the TGF-ß and BMP families of morphogenetic factors
• Discusses basic research before moving on to the future clinical role of the TGF-ß3 isoform, including research in clinical contexts

The effects of the bone morphogenetic proteins (BMPs) family on bone formation are well documented, but the transforming growth factor (TGF)-beta (ß) isoforms are much less studied. The product of 20 years of study, Induction of Bone Formation in Primates: The Transforming Growth Factor-ß3 sums up editor Ugo Ripamonti’s research into the osteogenic activity of the three mammalian TGF-ß isoforms, particularly in primates. It explores how the mammalian TGF-ß isoforms have the potential to shed light on the apparent redundancy of bone induction signaling.
The book unearths the profound and important bone inductive activity of the TGF-ß3isoform. It includes accounts of extensive research in non-human primates from craniofacial tissue regeneration, heterotopic tissue induction, and chapters on periodontal tissue regeneration and synergistic induction of bone formation. It also discusses the future clinical role of the TGF-ß isoform, including in human studies.
This book contributes to the fascinating history of BMP and TGF-ß research at the intersection of molecular biology, tissue induction, bone regeneration, and craniofacial surgery. It provides a revolutionary awakening to new possibilities in skeletal reconstruction, tissue engineering, and molecular and cellular biology.

Author
Ugo Ripamonti is a leader in the field of tissue induction and differentiation in postnatal osteogenesis. His principal achievement is the demonstration of the induction of bone formation in non-human primate models. In 1994 he was appointed professor at the Medical School University of the Witwatersrand in South Africa, leading its newly founded Bone Research Laboratory. He has written more than 200 publications, given more than 120 invited lectures at international symposia, and organized a series of workshops and symposia at the World Biomaterials Congresses and the International Conferences on Bone Morphogenetic Proteins.

Table of Contents
• Introduction: The Discovery of Redundancy and the Bone Induction Prerogative of the Three Mammalian Transforming Growth Factor-ß Isoforms
• Regenerative Medicine, the Induction of Bone Formation, Bone Tissue Engineering, and the Osteogenic Proteins of the Transforming Growth Factor-ß Supergene Family
• Rapid Induction of Bone Formation by the Transforming Growth Factor-ß3Isoform
• Induction of Bone Formation by the Mammalian Transforming Growth Factor-ßs: Molecular and Morphological Insights
• Regeneration of Mandibular Defects in Nonhuman Primates by the Transforming Growth Factor-ß3 and Translational Research in Clinical Contexts
• Synergistic Induction of Bone Formation by Relatively Low Doses of Transforming Growth Factor-ß1 and -ß3 in Binary Application with Recombinant Human Osteogenic Protein-1
• Induction of Periodontal Tissue Regeneration by Recombinant Human Transforming Growth Factor-ß3 with and without Myoblastic/Pericytic Stem Cells in Papio ursinus